assay

In order to investigate the regulatory mechanism of LIP genes at transcriptional level, 6 DNA fragments subcloned from 5′ upstream sequences of LIP genes ( GLG 3 and GLG 6) were obtained, then gel mobility shift assay was carried out to screen DNA fragment(s) with ability to be bound specifically by proteins isolated from P.

为研究ＬＩＰ基因的转录调控机理， 对ＬＩＰ基因（ ＧＬＧ３ 和ＧＬＧ６） 的５′端上游序列进行亚克隆， 获得６ 个亚克隆ＤＮＡ 片段， 然后应用凝胶迁移率变动分析技术筛选能与菌体蛋白质专一性结合的ＤＮＡ片段。

Furthermore, electrophoretic mobility shift assay(EMSA) showed that DIG-ddUTP labeled DNA sequences containing ezrin key elements could bind nuclear extracts from lung cancer cells to form DNA-protein complex, and the bindings to Sp1 and AP-1 sites by rhSp1 and rhAP-1, respectively, were specific.

其次,利用凝胶电泳迁移率变动分析证明,肺癌细胞核蛋白提取物能够与ezrin基因含有关键顺式作用元件的DNA序列结合,形成DNA-核蛋白复合物,而且Sp1结合位点和AP-1结合位点与重组蛋白rhSp1和rhAP-1的结合具有位点特异性。

They both showed bindings to the cbh1 promoter fragment(-304 bp to -18 bp) by electrophoresis mobility shift assays,suggesting ACEI a.

凝胶迁移率移动试验表明ACEI和Xyr1的DNA结合区均可与纤维素酶基因cbh1启动子的287 bp序列特异性结合。