By using a doubled haploid population(DH) containing 67 DH lines derived from anther culture of F1 cross between Balilla (japonica) and Nanjing 11 (indica), an RFLP map was constructed, which consisted of 131 RFLP markers covering all 12 linkage groups at an average distance 14.
A double haploid (DH) population with 132 plant lines and a recombination inbred lines (RIL) with 152 plant lines were constructed through one step anther culture and continuous self pollination of the hybrid between 02428 (japonica) and Teqing (indica), respectively.