Two order Thalassiosirales-specific primers were designed with their coverage and specificities determined based on the sequences available in GenBank and tested through selective amplification of 18S ribosomal RNA gene(rDNA)fragments of the microalgae in Thalassiosirales from total DNA of seawater plankton.
A copepod-specific primer was designed and used to amplify the 18S ribosomal RNA gene fragments of Jiaozhou Bay plankton copepod in combination with the universal primer of eukaryotes.
Methods Two bacterial isolates recovered randomly from the blood of one patient and one pig were analyzed for homogeneities by comparison with 16S rRNA gene sequences in the GeneBank.