nuclear reprogramming

We tried to analyse the factors such as the sort and the optimum cell cycle of the donor cell,the cytoplasm of the receiver,the nuclear reprogramming and the genomic imprinting of the reconstructed oocytes.

本文就供核细胞的种类、分化程度及其所处细胞周期、受核胞浆、核质相互作用、供体核与受体卵子细胞周期的同期化、核重排、基因印记、核移植技术和培养条件等方面分析影响核移植成功率的因素。

?Methods Using the total RNA extracted from fresh rat hippocampus as template,the cDNA of PDZ1 was amplified by RT-PCR;the cDNA obtained was subsequently cloned into the shuttle vector pAdTrack-CMV(CLONTECH),and then,the sequence of the product was determined.

目的对Ad-PDZ1-GFP穿梭重组体与骨架载体进行原核重组和真核包装。

Influencing factors of nuclear reprogramming after somatic cell nuclear transfer;

体细胞核移植后核重编程的影响因素

The developmental course of cell-free systems, material conditions of nuclear reconstitution and main progresses on development of sperm nucleus and nuclear reconstitution in cell-free systems were introduced and discussed in this paper.

本文就非细胞体系的发展历程、非细胞体系中细胞核重建的物质条件、非细胞体系中精核发育及核重建研究的主要进展进行了介绍和讨论。